Drug resistance of lactic acid bacteria and analysis of gyrA gene in milk of pastoral area of ​​Damaoqi, Inner Mongolia

Inner Mongolia Damaoqi pastoral area is rich in lactic acid bacteria, which are widely used in food, feed and pharmaceutical industries. Lactic acid bacteria have a long history of safe use. In recent years, studies have shown that the use of antibiotics in medicine, feed and other industries has led to the growing problem of microbial resistance, which has caused people to face severe challenges in dealing with microbial diseases. Studies have shown that nearly 50 to 70% of lactic acid bacteria in clinically isolated bacteria show resistance to fluoroquinolones.

Lactic acid bacteria showed high resistance rates to the four antibacterial drugs of quinolones, ofloxacin, ciprofloxacin, norfloxacin and aminoglycoside streptomycin, but there were also some differences. The four antibacterial drugs blindly study the drug resistance prevention and control and even the production practice without clear the drug resistance mechanism, which will cause uncertainty in the follow-up work. One of the mechanisms of lactic acid bacteria resistance is mediated by mutation of the topoisomerase gene. Type II topoisomerase (mainly DNA gyrase) is the target of quinolones and other drugs. One of the components of DNA gyrase is gyrA gene coding. A subunit. The purpose of this paper is to study the gyrA gene analysis of the resistance and resistance mechanism of lactic acid bacteria to the main antibacterial agents of ofloxacin, ciprofloxacin, norfloxacin and streptomycin in the milk of the pasture area of ​​Damaoqi, Inner Mongolia, for the selection of lactic acid bacteria. Breeding provides a reference for the direction and mechanism of drug resistance.