L-arabinose inhibits the increase of body fat caused by high fat diet in rats

L- arabinose inhibits the increase of body fat caused by high fat diet in rats
Yang Qing, Tan Zhuangsheng, Tong Guohui, Zhang Wei, Zheng Shan
(Beijing Institute of Disease Control and Prevention, Beijing 100013, China)
Key words: L-arabinose; high fat diet; increased body fat
As a healthy food additive, L-arabinose has been widely used in the food processing industry. It has been approved by the US Food and Drug Administration (FDA) and the Japanese Ministry of Health and Welfare for inclusion in health food additives. The American Medical Association will also L- Arabinose is listed as a nutritional supplement or over-the-counter for anti-obesity agents. Studies have shown that L-arabinose can inhibit the activity of invertase in the intestine non-competitively, regulate the blood sugar effect after ingesting sucrose, thereby inhibiting excessive sucrose conversion to body fat, and preventing and treating obesity.
And high blood lipids and other diseases [1-2]. At the same time, it has been reported that L-arabinose can reduce the fat index of obese rabbits caused by high sugar and high fat [3]. However, the effect of L-arabinose on the accumulation of body fat in rats fed with high-fat diet was rarely reported. This study investigated the inhibitory effect of L-arabinose on body fat accumulation in rats induced by high-fat diet.
1 Materials and methods
1.1 Material L-arabinose (purity 98%, HPLC); high fat nutritional feed: 80% base feed, 10% lard, 10% egg yolk powder. Feed source: Laboratory Animal Center of Academy of Military Medical Sciences; feed certificate number: SCXK-(军)2007-005.
1.2 Animal clean male Wistar rats 50, weighing 140 ~ 172 g, provided by the Experimental Animal Center of the Academy of Military Medical Sciences, animal certificate number: SCXK- (Jun) 2007-004. Breeding environment: room temperature 20.0 ~ 22.5 ° C, relative humidity of 45.5% ~ 50.0%, single cage feeding. Animal Laboratory Certificate No.: SYXK (Beijing) 2008-0001.
1.3 Methods 50 healthy adult male rats were randomly divided into 5 groups according to their body weight, namely, negative control group, obesity model group and 3 test substance dose groups. According to the daily recommended intake of human body, 6 g/person. The daily dose was calculated in the low dose group (500 mg/kg), the middle dose group (1 000 mg/kg), and the high dose group (3 000 mg/kg), with 10 animals in each group. Except for the negative control group, the other groups were fed with high fat diet. Administering different doses of the test substance, two
The control group was given the same volume of deionized water by intragastric administration, and was administered by intragastric administration of 1 ml/100 g for 32 days and intragastric administration once a day. Fasting for 16 h before the end of the experiment, weighing the body and then dying and dissecting the abdominal testicles and peri-renal fat.
1.4 Observation index The body weight was weighed once a week and the food intake of the rats was recorded. After the experiment, the body fat weight (testicle and weight around the kidney) was weighed.
1.5 Statistical methods SPSS 11.0 statistical software package was used for data processing, and weight gain, food utilization and fat ratio of each group were calculated and compared. Statistical analysis was performed by one-way analysis of variance. All data were expressed as x珋±s, and the test level was α=0.05.
2 results
2.1 The effect of L-arabinose on body weight, food intake and food utilization rate in rats can be seen from Tables 1 and 2. The final body weight of rats in the high dose group of 3 000 mg/kg is lower than that of the obese model control group, and the difference is statistically significant ( P < 0.05). There was no significant difference in body weight between the other groups and the control group (P>0.05). There was no significant difference in weekly food intake and total food intake between the two groups (P>0.05). It is indicated that L-arabinose has no effect on the appetite of rats. The total food utilization rate of the 1 000 mg/kg middle dose group and the 3 000 mg/kg high dose group was significantly different from that of the obese model control group (P<0.05), indicating that under the same weight and high fat diet, The weight gain of the rats in the 1 000 and 3 000 mg/kg two-dose groups was slower than that in the obese model control group.
2.2 The effect of L-arabinose on body weight, body fat and liposome ratio in rats. It can be seen from Table 3 that the body fat weight and fat body ratio of the control group were higher than those of the negative control group, and statistically tested. The difference was statistically significant (P<0.01), indicating that the animal fat accumulation model was successfully manufactured. At the same time, the weight gain, body fat weight and liposome ratio of the animals fed the high-fat nutrient diet were lower than those of the obese model control group, and statistically tested, 3 000 mg/kg. There was a statistically significant difference between the high-dose group (P<0.05 or P<0.01). There was no significant difference between the low-dose 500 mg/kg group and the 1 000 mg/kg medium-dose group (P>0.05). It is indicated that the administration of L-arabinose at a dose of 3 000 mg/kg for 1 month can inhibit the increase of body fat caused by high-fat diet in rats.
3 discussion
A few days ago, L-arabinose has been approved by the Ministry of Health as a new resource food [4]. It has been reported in the literature that L-arabinose can not only effectively interfere with the blood glucose level caused by long-term high sugar and high fat, enhance the glucose tolerance of animals, and effectively inhibit the synthesis of body fat by effectively inhibiting the level and metabolism of blood sugar. Reduce the level of visceral fat index in the body [5]. It has also been reported in the literature that L-arabinose inhibits body fat accumulation caused by feeding high-sugar and high-fat diets in rabbits, which significantly inhibits serum triacylglycerol levels and effectively inhibits fat accumulation and lowers fat index [3]. The L-arabinose used in this experiment was prepared from rice and corn straw by chemical biological methods. In the experiment, rats were fed a high-fat diet to induce a fat accumulation model, and the experimental results were consistent with other animals. It is inferred that the standard nutrient ratio of standard feed is 60% for carbohydrates, 20% to 21% for crude protein, and 5% for crude fat, while the main nutrient content of high-fat nutrient feed is 80% for basic feed, 10% for lard and egg yolk powder. 10%. Therefore, carbohydrates account for 48% of high-fat nutrient feeds. L-arabinose can effectively inhibit the absorption of sucrose in the intestine, inhibit the activity of lipogenic enzymes and the level of serum triacylglycerol, and finally achieve the effect of reducing body fat [6] ]. A Japanese patent also proves that L-arabinose can reduce the weight growth rate of rats, and can reduce the proportion of body fat, which plays an important role in effective weight loss. Therefore, L-arabinose can inhibit the increase of body fat caused by high-fat diet in rats.
references
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[2] Eric PK, Mary AF, Boris US, et al. Utilization and transport of L-Arabinose by non-Saccharomyces yeasts [J]. Cellulose, 2009, 16(4): 729-741.
[3]Han Wei, Wu Hanzhou, Xie Yupeng, et al. Study on the effect of L-arabinose on reducing fat index in obese rabbits caused by high sugar and high fat[J].中国医药导刊,2009,11(9):1528- 1530.
[4]TANG Xiaodan,ZHANG Wei,LIN Bingchang.Progress in the preparation and application of L-arabinose[J].Journal of Anhui Agricultural Sciences,2009,37(10):4643-4644,4646.
[5]Zhou Kefu, Han Wei, Zhang Zhongying, et al. Effects of L-arabinose on body weight and glucose tolerance in high glucose and high fat fed mice[J].中国医药导杂志,2009,11(1):86 -89.
[6] Groop L. Pathogenesis of type 2 diabetes: the relative contribution of insulin resistance and impaired insulinsecretion [J]. Int J ClinPract, 2000 (Sup. 1): 3-13.·316·Toxicology Journal August 2010 Volume 24, Issue 4 J Toxicol August 2010 Vol.24 No.4

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