Treatment of Rice Bakanae Disease with Prochloraz to Prevent Drug Resistance

Bakanae disease is an important disease on rice. The benzoimidazole drug represented by carbendazim used to be the main agent for the prevention and treatment of the disease. As the bastard disease pathogen produces resistance to carbendazim, it is used alone. Decline in efficiency. Prochloraz is an imidazole bactericide. In recent years, it has been used to control bakanae disease in rice and has achieved better control effects. But at present, there is also a phenomenon that the control effect is reduced in some areas.
In response, researchers from the College of Agronomy and Biotechnology at the China Agricultural University and researchers from the Ministry of Agriculture's Institute of Pesticides used the mycelium growth rate and microscopic observation methods to study the growth of susceptible strains and resistant mutant strains of rice bakanae against rice bakanae. Development and morphology of mycelial growth. The results showed that the treatment of 0.5 mg/l prochloraz solution completely inhibited the growth of mycelia of sensitive strains, and could not inhibit the growth of mycelia of resistant strains with different levels of resistance; treatment with 20 mg/l prochloraz solution could completely inhibit the parent The spore germination of susceptible strains showed inhibition rates of 84.1%-89% and 58.5%-65.8% for the spore germination and shoot elongation of different resistance mutants, respectively, indicating that the resistant mutant strains were in the mycelium growth, spore germination, The susceptibility of the buds to elongation and other stages has been reduced to varying degrees compared to the parental susceptible strains. After 12 hours treatment of hyphae with 10 mg/l prochloraz solution, the mycelium tip of the parent susceptible strains swelled and deformed, the mycelium tip of the resistant mutants swelled, but there were many small branches on the hyphae, indicating that the agents were antagonistic The inhibitory effect of secondary mutant hyphae was not obvious.
The above test results indicate that rice bakanae pathogens have potential drug resistance risks to prochloraz. It is recommended that the drug be used interchangeably with dithiocyanate methane, trichloroisocyanuric acid and other mechanisms with different mechanisms to delay or Avoid drug resistance.

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