The correct choice of microinjection system
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Microinjection refers to the injection of a small amount of liquid using a metal core or a glass capillary head. For example, genetic material is transfected into living cells, or the drug is introduced into the brain or animal brain, or liquid is injected into the muscle. Typically, microinjection is done under a microscope and a stereo positioner may be required.
WPI offers a variety of microinjector pumps with special syringes, stereotaxes, glass capillaries and needles. Here we will focus on several microinjection pumps. Which syringe pump you choose to install depends on whether the liquid you are injecting is an integer, the size of the injection, the size of the needle selected, or the size of the glass dripper. The following description will be made in accordance with the minimum volume order in which the syringe can be injected.
NanoFil TM
NanoFil is the world's smallest dead space (2.7μL) injection system that can be used either manually or with MMP, DMP or UMP3 pump systems. RPE (Retinal Pigment Epithelial Kit) and I/O (Intra-injection Kit) contain two SilFlex tubes with a dead volume of only 2.74 μL. If Nanofil is used with an injection needle, the dead volume is only 1.5 nL. As small as the syringe itself. When it is not desired to refill, the syringe is very good for small volume injection and syringe filling. The common use is eye injection. We currently offer two syringes (10μL and 100μL) in different sizes, and the syringe is also equipped with a Silflex tube. Silflex is a flexible and durable glass tube embedded in plastic. The tube has a very small diameter for small volumes, while the length of 35 cm allows the manual injector to move freely. NanoFil's other options include two injection kits, IO-KIT for intraocular injection and RPE-KIT for retinal pigment epithelial injection.
Pneumatic PicoPumps
The pneumatic skin lift pumps PV820 and PV830 are designed to simplify intracellular injections, and you can perform repeated microinjections from picoliter to nanoliter. The PV820 can only provide injection pressure and maintenance pressure. The maintenance pressure can prevent the dripper liquid from pumping back due to capillary siphoning. In addition, the PV830 has a vacuum pressure so that one capillary can safely hold the cells and allow the operator to simultaneously inject another capillary. The amount of injection is determined by the inner diameter of the glass tube drip, the pressure and the injection time. The syringe pump can inject liquid volumes ranging from pico to microliters.
Nanoliter 2010
The Nanoliter 2010 system is an upgraded version of the current Nanoliter 2000, and Nanoliter 2000 will no longer be available in the future. The system is perfect for oocyte injections and injections in the range of 2.3 to 69 nanoliters. It provides a fast, accurate injection of an integral multiple of 2.3 nanoliters, which works through a glass capillary dripper. The glass capillary was backfilled with vegetable oil, and then the vegetable oil was extruded, followed by filling the glass capillary with the injection. The maximum volume is limited by the size of the glass capillary dripper. Nanoliter 2010 can be used with the Micro4 controller.
UMP3
UMP3 is the first microinjection pump on the market, and is the best choice for brain stereotactic injection after improved design. The UMP3 is also an automated syringe pump that can be connected to a 0.5 microliter to 1 milliliter syringe. The minimum volume is 5-25 nanoliters when using a 10 microliter syringe. Intuitively, UMP3 combined with Micro4 can achieve intelligent control effects. For high-precision injection, the syringe pump has excellent sealing performance. It can also be used in the M3301 micromanipulator and DC3001 automatic locator or any stereo positioning operation.
Manual Microsyringe Pumps
Both the MMP (Manual Microinjection Pump) and DMP (Digital Microinjection Pump) systems are manual microinjection pumps. They all use a manual micro-operator to inject liquid, and the micro-operator in DMP comes with a digital display, both using a glass micro-dropper for injection, all of which are simple, manually operated perfusion systems.
Dual Microiontophoresis Current Generator
The bidirectional microion electrophoresis current generator (Cat. No. SYS-260) is a battery-powered instrument with an electronic isolator designed for ion electrophoresis of dyes, drugs and charged substances from microtiter heads. In normal applications, two current generators provide two different current operations in parallel; one of the currents prevents the material in the droplet head from spreading out (maintaining current or clamping current), while the other current actively discharges the charged material. .