Mouse cysteine ​​protease-3elisa kit instruction manual
Natural Sweeteners
ï¬The sufficient capacity of GMP standard workshop covers all customers` demand.
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Elisa kit use purpose
1 30 times concentrated washing solution 20ml × 1 bottle 7 stop solution 6ml × 1 bottle
2 enzyme standard reagent 6ml × 1 bottle 8 standard (160 pmol / L) 0.5ml × 1 bottle
3 Enzyme label coating plate 12 holes × 8 strips 9 standard dilutions 1.5ml × 1 bottle
4 sample dilution 6ml × 1 bottle 10 instructions 1
5 developer A liquid 6ml × 1 bottle 11 sealing film 2 sheets
6 developer B liquid 6ml × 1 bottle 12 sealed bag 1
1. The specimens should be extracted as soon as possible after collection, and the extraction should be carried out according to the relevant literature. The experiment should be carried out as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 °C, but repeated freezing and thawing should be avoided.
2. Samples containing NaN3 could not be detected because NaN3 inhibited horseradish peroxidase (HRP) activity.
Dilution of Standards: This kit provides a standard of the original standard, which can be diluted in a small tube according to the following chart.
Add 150 μl of standard dilution to the original standard of 150 μl of 80 pmol/L No. 5 standard
40 pmol/L No. 4 Standard 150 μl of No. 5 Standard Add 150 μl Standard Diluent
20 pmol/L No. 3 Standard 150 μl of Standard No. 4 Add 150 μl of Standard Diluent
10 pmol/L No. 2 Standard 150 μl of No. 3 Standard Add 150 μl Standard Diluent
5 pmol/L No. 1 Standard 150 μl of No. 2 Standard Add 150 μl Standard Diluent
Incubation: The plate was sealed with a sealing film and incubated at 37 ° C for 30 minutes.
Solution: 30 times concentrated washing solution is diluted with distilled water 30 times and then washed. Carefully remove the sealing film, discard the liquid, dry it, fill each well with washing liquid, let stand for 30 seconds, then discard it, repeat 5 Times, shoot dry.
Add enzyme: 50 μl of enzyme labeling reagent was added to each well, except for blank wells.
Incubation: The operation is the same as 3.
Washing: The operation is the same as 5.
Color development: Add 50 μl of color developer A to each well, then add 50 μl of color developer B, gently shake and mix, and develop color at 37 ° C for 15 minutes.
Termination: 50 μl of stop solution was added to each well to terminate the reaction (in this case, the blue color turned yellow).
Measurement: The absorbance (OD value) of each well was measured sequentially with a blank air conditioner of zero and a wavelength of 450 nm. The measurement should be carried out within 15 minutes after the addition of the stop solution.
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Characteristic of NovoSweet Natural Sweeteners:
ï¬Natural, it is extracted from plant and fruit.
ï¬High in sweet, Low in calorie.
ï¬Low cost, they are widely used in food, beverage, medicine, wine etc.
ï¬ISO9000&22000/Kosher/Halal/Organic certified.
ï¬Complete products line for all series of the extractions.
