The role of Na+/H+ transporter in the plasma membrane of wheat roots and its vacuoles in salt tolerance

The role of Na+/H+ transporter in the plasma membrane of wheat roots and its vacuoles in salt tolerance

The cytosolic Na ⁺ /K ⁺ ratio is important in the salt-tolerant process of plants. Sweet soil plants (such as wheat) can prevent the cytosolic Na ⁺ /K ⁺ ratio from being too high by increasing the Na ⁺ efflux of the roots. The Na ⁺ efflux is a process of energy dissipation, and the Na ⁺ /H ⁺ transporter on the plasma membrane is considered to be an active efflux system that transports Na ⁺ out of plant cells. Although this active efflux system is significant in salt tolerance, the relationship between its activity and activity and salt tolerance is still poorly understood, and there is a lack of Na ⁺ /H ⁺ transporters for any plant species. A method for rapid and convenient quantitative assessment of activity.

In March 2011, Australian researchers studied the sodium ion flux and internal sodium ion concentration in different varieties of wheat by non-invasive micro-measurement (MIFE), laser confocal, and scanning electron microscopy. The study found that the active efflux of sodium ions in wheat root cells is regulated by the SOS1 gene family and is powered by the plasma membrane proton pump. The salt-tolerant wheat variety Kharchia 65 has the highest efflux activity, and the laser confocal results show that Kharchia 65 can more partition the Na ⁺ zone into the vacuole. These results are consistent with higher expression levels of SOS1 (Na ⁺ /H ⁺ transporter on the plasma membrane) and NHX1 (Na ⁺ /H ⁺ transporter on the tonoplast) in this breed. In salt-sensitive wheat, a large amount of Na ^{+ is} localized in the cytoplasm of the root cell, which destroys the Na ⁺ /K ⁺ balance of the cell.

The above results indicate the relationship between Na ⁺ distribution and salt tolerance in wheat, indicating the important role of cytosolic Na ⁺ efflux in wheat salt tolerance, and a quantitative measure of transporter salt tolerance. A convenient way to contribute.