How the horizontal rotor of the centrifuge works
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1. How to install the basket 2. Wall effect (welleffects)
When the split rotor is rotated in the centrifuge, the centrifugal force acts in a radial direction, and the horizontal section is a symmetrical fan shape. Only the particles on the centerline of the centrifuge tube are in the same direction as the centrifugal force field, and the particles at other locations are in the sedimentation process. It will hit the pipe wall and settle along the pipe wall. The wall effect in the split rotor is not as severe in the angle rotor.
The particle sedimentation path in the centrifuge tube is perpendicular to the axis of rotation. The particles in the center of the initial sample zone will settle radially. However, the particles near the pipe wall will collide with the pipe wall first, and the distance from the pipe wall will reach the bottom of the pipe. The order is different, and the widening of the zone will also cause particle accumulation, blockage, convection and other anomalies.
3. Balance of the rotor (balancingoftherotor)
For the use of centrifuges, balancing the rotor is the key to good separation. The balance method for the number of different baskets (test tubes) is shown in the figure. The weight balance error of the shaft corresponding to the test tube must be kept within 0.1g, and the basket must be placed on the rotor body in an axisymmetric manner. Some centrifuges require that all baskets must be installed.
4. Split the rotor separation process a First prepare the gradient, lay the sample, hang (mount) the basket on the rotor (the basket is vertically suspended when the centrifuge is not rotating); when the b rotor accelerates, the basket begins When the speed reaches 800r/min or more, the basket and the rotating shaft of the rotor are at a horizontal position of 90° without gradient reorientation; c under the action of the centrifugal force field, the particles in the sample settle to the bottom of the tube, according to The difference between the sedimentation coefficient or the buoyancy density is separated into discontinuous zones; the d rotor is decelerated, the basket returns to the original vertical position, and no reorientation occurs in the test tube. 5. In the radius-volume relationship of the split rotor, rt The distance between the center of rotation and the top of the liquid level.
If you consider the impact on the hemisphere bottom, you can use the following formula:
Where b is the total volume of the solution contained in the tube; V is the volume of the solution from the meniscus to r; r is the distance between the shafts, Wt is the thickness of the tube bottom; t is the thickness of the tube wall; D is the centrifuge tube Hole radius; Rmax is the large radius of the rotor.
6. Use a short-thickness centrifuge tube for iso-density centrifugation and a slender centrifuge tube for centrifugation at a rate zone. These are the common techniques for preparing centrifuges for analysis. The split rotor is also used in differential centrifuge separation in a few cases. Since the radius-volume relationship of the split rotor is relatively simple and easy to form an equal dynamic gradient, it is the main method for determining the sedimentation coefficient by the preparation machine.