General operation method of solid phase extraction column
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General operation of solid phase extraction
Reversed phase extraction column operation
Normal phase extraction column operation
Ion exchange extraction column
Extract from polar matrix
Non-polar sample
Extracted from a non-polar matrix
Polar sample
Extracting charged samples
activation
The extraction column is rinsed with 3-5 ml of methanol;
Rinse the extraction column with 3-5 ml of deionized water (or buffer) and keep the extraction column moist
3-5 ml of non-polar solvent
Leaching extraction column
Rinse the extraction column with 3-5 ml of deionized water/or low ionic strength buffer
Loading
Make the sample at the right flow rate
Uniformly passing through the extraction column
Pass the sample evenly through the extraction column at a lower flow rate
Cleaning
The extraction column of the adsorbed sample is eluted with 5 ml of a solvent of the appropriate polarity (commonly used is pure water, buffer, water/organic solvent mixture)
Extraction column for eluting the sample with 5 ml of the appropriate non-polar solvent
5 ml deionized water / or low ionic strength buffer rinse column
Elution
The sample to be tested is eluted in batches with 1-5 ml of non-polar solvent to the collection container.
The sample to be tested is eluted in batches with 1-5 ml of polar solvent to the collection container.
The sample to be tested is eluted into the collection container with 1-5 ml of high ionic strength buffer.