Analysis of Triton X-100 (polyethylene glycol octyl phenyl ether) using ultra-high performance convergent chromatography
Introduction Commonly used in the detection methods are: HPLC, SFC and GC. GC and HPLC methods are very time consuming. Non-UV absorbing surfactants need to be derivatized to use HPLC methods. In some cases, these two methods are still not possible. Baseline separation of oligomers. For more information on the products used in this application, please click: http://?cid=134658367 We provide wide range of herb tea cut, like echinacea tea, nettle leaf tea, dandelion root tea, valerian root tea and red raspberry leaf tea. We can provide blend herbal tea according to formula of customer and we also provide formula according to needs of customers. As one stop shop store of natural ingredients, tea bags like pyramid tea bag and string tea bag can be processed too. Private label is acceptable. Herb Tea Cut,Echinacea Tea,Nettle Leaf Tea,Dandelion Root YT(Xi'an) Biochem Co., Ltd. , https://www.ytnutra.com
Nonionic surfactants such as Triton X-100 are used in cosmetics, industrial materials and many other products. The difference in ethoxy chain length affects the viscosity, polarity and other properties of the mixture, so its composition must be Detection.
Test Conditions <br>System: ACQUITY UPC 2 TM
Detection: Photodiode Array (PDA)
PDA 3D channel: PDA, 210 to 400 nm;
PDA 2D channel: 222 nm at 4.8nm resolution (compensated for 380 to 480 nm)
Column: ACQUITY UPC 2 BEH, 2.1 x 50 mm, 1.7 μm
Mobile phase A: CO 2
Mobile phase B: methanol wash solvent: 70:30 methanol/isopropanol separation method: starting gradient 2% B, rising to 35% in 1.25 minutes, then returning to 2% B in 5 minutes.
Flow rate: 2.0 mL/min
UPC 2TM Manager: 1500 psi
Column temperature: 40 °C
Injection volume: 1.0 μL
Running time: 2 min
Sample: 10 mg/mL Triton X-100 Isopropanol Solution Software: EmpowerTM 3
in conclusion
Ultra-High Performance Convergence Chromatography (UPC 2TM ) provides a fast and efficient separation method for Triton X-100 with a very high resolution baseline of 2 minutes at temperatures lower than those tested by GC or conventional SFC methods. Separation of about 20 oligomers makes UPC 2 more suitable for thermally unstable compounds, which significantly reduces the use of toxic solvents and reduces the cost of solvent use compared to normal phase HPLC. 